Intracellular calcium dynamics of lymphatic endothelial and muscle cells co-cultured in a Lymphangion-Chip under pulsatile flow.

ABSTRACT: The lymphatic vascular function is regulated by pulsatile shear stresses through signaling mediated by intracellular calcium [Ca²⁺]_i. Further, the intracellular calcium dynamics mediates signaling between lymphatic endothelial cells (LECs) and muscle cells (LMCs), including the lymphatic tone and contractility. Although calcium signaling has been characterized on LEC monolayers under uniform or step changes in shear stress, these dynamics have not been revealed in LMCs under physiologically-relevant co-culture conditions with LECs or under pulsatile flow. In this study, a cylindrical organ-on-chip platform of the lymphatic vessel (Lymphangion-Chip) consisting of a lumen formed with axially-aligned LECs co-cultured with transversally wrapped layers of LMCs was exposed

SUBMITTER: Selahi A 

PROVIDER: S-EPMC9512566 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature