Novel lineages of single-stranded DNA phages that coevolved with the symbiotic bacteria Rhizobium.

ABSTRACT: This study describes novel single-stranded DNA phages isolated from common bean agriculture soils by infection of the nitrogen-fixing symbiotic bacteria Rhizobium etli and R. phaseoli. A total of 29 phages analyzed have 4.3-6 kb genomes in size and GC 59-60%. They belong to different clades unrelated to other Microviridae subfamilies. Three-dimensional models of the major capsid protein (MCP) showed a conserved β-barrel structural "jelly-roll" fold. A variable-length loop in the MCPs distinguished three Rhizobium microvirus groups. Microviridae subfamilies were consistent with viral clusters determined by the protein-sharing network. All viral clusters, except for Bullavirinae, included mostly microviruses identified in metagenomes from distinct ec

SUBMITTER: Van Cauwenberghe J 

PROVIDER: S-EPMC9512667 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature