Increased mast cell activation in eosinophilic chronic obstructive pulmonary disease.

ABSTRACT:

Objectives

A subset of chronic obstructive pulmonary disease (COPD) patients have increased numbers of airway eosinophils associated with elevated markers of T2 inflammation. This analysis focussed on mast cell counts and mast cell-related gene expression in COPD patients with higher vs lower eosinophil counts.

Methods

We investigated gene expression of tryptase (TPSAB1), carboxypeptidase A3 (CPA3), chymase (CMA1) and two mast cell specific gene signatures; a bronchial biopsy signature (MC_bb) and an IgE signature (MC_IgE) using sputum cells and bronchial epithelial brushings. Gene expression analysis was conducted by RNA-sequencing. We also examined bronchial biopsy mast cell numbers by immunohistochemistry.

Results

There was

SUBMITTER: Higham A 

PROVIDER: S-EPMC9512688 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature