Actomyosin contractility as a mechanical checkpoint for cell state transitions.

ABSTRACT: Cell state transitions induced by mechano-chemical cues result in a heterogeneous population of cell states. While much of the work towards understanding the origins of such heterogeneity has focused on the gene regulatory mechanisms, the contribution of intrinsic mechanical properties of cells remains unknown. In this paper, using a well-defined single cell platform to induce cell-state transitions, we reveal the importance of actomyosin contractile forces in regulating the heterogeneous cell-fate decisions. Temporal analysis of laterally confined growth of fibroblasts revealed sequential changes in the colony morphology which was tightly coupled to the progressive erasure of lineage-specific transcription programs. Pseudo-trajectory constructed using unsupervised diffusion analysis of th

SUBMITTER: Venkatachalapathy S 

PROVIDER: S-EPMC9512847 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature