Plasma-derived exosomal mRNA profiles associated with type 1 diabetes mellitus.

ABSTRACT:

Background

Exosomes carry various types of transcripts, such as messenger RNAs (mRNAs), and play an important role in mediating cell-to-cell communication, thus influencing multiple physiological and pathological processes. However, the role of exosomal mRNAs in T1DM is largely unknown. Here, we aimed to identify the plasma-derived exosomal mRNA expression profiles in T1DM and to explore their potential biological functions in T1DM.

Materials and methods

Plasma-derived exosomes were isolated from 10 patients with T1DM and 10 age- and sex-matched control subjects by size exclusion chromatography methods. Transmission electron microscopy, nanoparticle tracking analysis, and western blot analysis confirmed the presence of exosomes. The exosomal mRNAs were analyzed using the Ill

SUBMITTER: Fan W 

PROVIDER: S-EPMC9513134 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature