Increased atherosclerotic plaque in AOC3 knock-out in ApoE^-/- mice and characterization of AOC3 in atherosclerotic human coronary arteries.

ABSTRACT:

Introduction

Amine oxidase copper containing 3 (AOC3) displays adhesion between leukocytes and endothelial cells and enzymatic functions. Given its controversial role in atherogenesis, we proposed to investigate the involvement of AOC3 in the formation of atherosclerotic plaques in ApoE^-/-AOC3^-/- mice and human coronary arteries.

Methods

Lesions, contractile markers, and AOC3 were studied in aortic tissues from 15- and 25-week-old mice and different stages of human coronary atherosclerotic arteries by immunohistochemistry (IHC) and/or western blot. Human VSMCs, treated or not with LJP1586, an AOC3 inhibitor, were used to measure differentiation markers by qPCR. AOC3 co-localization with specific cell markers was studied by using confocal microscopy in m

SUBMITTER: Filip A 

PROVIDER: S-EPMC9513161 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature