Characterization of chicken p53 transcriptional function via parallel genome-wide chromatin occupancy and gene expression analysis.

ABSTRACT: The tumor suppressor p53, which acts primarily as a transcription factor, can regulate infections from various viruses in chickens. However, the underlying mechanisms of the antiviral functions of chicken p53 (chp53) remain unclear due to the lack of detailed information on its transcriptional regulation. Here, to gain comprehensive insights into chp53 transcriptional regulatory function in a global and unbiased manner, we determined the genome-wide chromatin occupancy of chp53 by chromatin immunoprecipitation, which was followed by sequencing and chp53-mediated gene expression profile by RNA sequencing using chemically immortalized leghorn male hepatoma (LMH) cells with ectopic expression of chp53 as the model. The integrated parallel genome-wide chromatin occupancy and gene expression an

SUBMITTER: Chen Z 

PROVIDER: S-EPMC9513273 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature