Human differentiated eosinophils release IL-13 in response to IL-33 stimulation.

ABSTRACT:

Objective

Eosinophils are hallmarks in allergic type 2 inflammation and are known to release cytotoxic granule proteins that contribute to inflammation. Eosinophils develop in the bone marrow from hematopoietic stem cells and once mature, have a limited lifespan in culture, making them difficult to study ex vivo. IL-33 has increasingly been shown as a key regulator of type 2 inflammation via signaling through its receptor, ST2. The present study was conducted to detail a method of eosinophil differentiation from hematopoietic stem cells and determine the response to IL-33.

Methods

CD34+ and CD14+ cells were isolated from donor apheresis cones and differentiated into eosinophils or macrophage controls, respectively. Morphologic, transcriptional and protein analy

SUBMITTER: Uchida AM 

PROVIDER: S-EPMC9513478 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature