Timed Notch Inhibition Drives Photoreceptor Fate Specification in Human Retinal Organoids.

ABSTRACT:

Purpose

Transplanting photoreceptors from human pluripotent stem cell-derived retinal organoids have the potential to reverse vision loss in affected individuals. However, transplantable photoreceptors are only a subset of all cells in the organoids. Hence, the goal of our current study was to accelerate and synchronize photoreceptor differentiation in retinal organoids by inhibiting the Notch signaling pathway at different developmental time-points using a small molecule, PF-03084014 (PF).

Methods

Human induced pluripotent stem cell- and human embryonic stem cells-derived retinal organoids were treated with 10 µM PF for 3 days starting at day 45 (D45), D60, D90, and D120 of differentiation. Organoids were collected at post-treatment days 14, 28, and 42 and analyzed for prog

SUBMITTER: Chew SH 

PROVIDER: S-EPMC9513742 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature