Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo.

ABSTRACT: Characterization of molecular events as cells give rise to tissues and organs raises a potential to better understand normal development and design efficient remedies for diseases. Technologies enabling accurate identification and quantification of diverse types and large numbers of proteins would provide still missing information on molecular mechanisms orchestrating tissue and organism development in space and time. Here, we present a mass spectrometry-based protocol that enables the measurement of thousands of proteins in identified cell lineages in Xenopus laevis (frog) embryos. The approach builds on reproducible cell-fate maps and established methods to identify, fluorescently label, track, and sample cells and their progeny (clones) from this model of vertebrate development. After c

SUBMITTER: Baxi AB 

PROVIDER: S-EPMC9513837 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature