Improved Bst DNA Polymerase Variants Derived via a Machine Learning Approach.

ABSTRACT: The DNA polymerase I from Geobacillus stearothermophilus (also known as Bst DNAP) is widely used in isothermal amplification reactions, where its strand displacement ability is prized. More robust versions of this enzyme should be enabled for diagnostic applications, especially for carrying out higher temperature reactions that might proceed more quickly. To this end, we appended a short fusion domain from the actin-binding protein villin that improved both stability and purification of the enzyme. In parallel, we have developed a machine learning algorithm that assesses the relative fit of individual amino acids to their chemical microenvironments at any position in a protein and applied this algorithm to predict sequence substitutions in Bst DNAP. The top predicted v

SUBMITTER: Paik I 

PROVIDER: S-EPMC9514386 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature