First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia.

ABSTRACT:

Background

Neospora caninum has been documented to infect most domestic wildlife but is known to primarily infect dogs and cattle and is considered an important cause of abortion in camels.

Objective

The aim of this study was to estimate the molecular detection of Neospora caninum in tissues of naturally infected camelids.

Methods

Brain, tongue (bottom and tip) and masseter muscles from 35 slaughtered camelids from Tataouine and Médenine regions were collected (n = 140 samples). PCR was used to amplify and detect N. caninum DNA in tissues samples followed by sequencing of some PCR products. A phylogenetic tree was then constructed to compare the partial sequences of the ITS1 gene with GenBank sequences. Histopathology examination was used to detect Neospora spp. cysts

SUBMITTER: Amdouni Y 

PROVIDER: S-EPMC9514472 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature