Expression and distribution patterns of VEGF, TGF-β1 and HIF-1α in the ovarian follicles of Tibetan sheep.

ABSTRACT: Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and transforming growth factor β1 (TGF-β1 ) are multifunctional growth factors that play an important role in follicular growth and development. However, its biological function in the follicular development of Tibetan sheep at different stages has not been described. The purpose of this study was to investigate the effect of VEGF, TGF-β1 and HIF-1α expression and distribution on the development of follicles of different sizes. Immunohistochemistry (IHC), western blot (WB) and quantification real-time polymerase chain reaction (qRT-PCR) were used to detect the localisation and quantitative expression of VEGF, TGF-β1 and HIF-1α proteins and mRNA in small- (< 3 mm), medium- (3 mm < diameter < 5 mm)-, and large- (

SUBMITTER: Guo Y 

PROVIDER: S-EPMC9514499 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature