Evidence of bovine immunodeficiency virus: A molecular survey in water buffalo populations of Iran.

ABSTRACT:

Background

Bovine immunodeficiency virus (BIV) is a member of the Retroviridae family causing a progressive lifelong infection in cattle and buffaloes.

Objective

Despite the worldwide distribution of the virus, the studies concerning the prevalence of BIV in buffalo populations have not been conducted in Iran as yet.

Methods

The BIV proviral DNA was surveyed in 120 whole blood samples of water buffaloes in southwestern Iran. Nested PCR was employed to amplify a 298-bp fragment of the pol gene. The BIV Pol sequence was detected in 9.1% of the samples. Among PCR-positive samples, two amplified fragments were confirmed by nucleotide sequencing.

Results and conclusions

The studied sequences were completely identical to each other and had more than 98%-99% nucleotid

SUBMITTER: Keshavarz H 

PROVIDER: S-EPMC9514501 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature