Mitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos.

ABSTRACT: Studies of mitochondrial dynamics have identified an intriguing link between energy supply balance and mitochondrial architecture. This suggests that inappropriate culture conditions might inhibit mitochondrial functions, and affect embryonic development. Therefore, this study was conducted to determine whether in vitro culture (IVC) might affect mitochondrial function, distribution, organization (by Mitotracker Green), gene expression on RNA level (by qPCR), and protein expression and localization (by western blot and immunostaining) involved in regulation of mitochondrial functions. Mitochondria in 2-cell IVC embryos were less numerous compare to IN VIVO while the localization and distribution do not differ between the groups. Mitochondria of in vivo blastocysts formed elongated network

SUBMITTER: Czernik M 

PROVIDER: S-EPMC9515144 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature