Generation of a recombinant version of a biologically active cell-permeant human HAND2 transcription factor from E. coli.

ABSTRACT: Transcription factor HAND2 has a significant role in vascularization, angiogenesis, and cardiac neural crest development. It is one of the key cardiac factors crucial for the enhanced derivation of functional and mature myocytes from non-myocyte cells. Here, we report the generation of the recombinant human HAND2 fusion protein from the heterologous system. First, we cloned the full-length human HAND2 gene (only protein-coding sequence) after codon optimization along with the fusion tags (for cell penetration, nuclear translocation, and affinity purification) into the expression vector. We then transformed and expressed it in Escherichia coli strain, BL21(DE3). Next, the effect (in terms of expression) of tagging fusion tags with this recombinant protein at two different terminals was also

SUBMITTER: Haridhasapavalan KK 

PROVIDER: S-EPMC9515176 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature