Molecular mechanisms of steric pressure generation and membrane remodeling by disordered proteins.

ABSTRACT: Cellular membranes, which are densely crowded by proteins, take on an elaborate array of highly curved shapes. Steric pressure generated by protein crowding plays a significant role in shaping membrane surfaces. It is increasingly clear that many proteins involved in membrane remodeling contain substantial regions of intrinsic disorder. These domains have large hydrodynamic radii, suggesting that they may contribute significantly to steric congestion on membrane surfaces. However, it has been unclear to what extent they are capable of generating steric pressure, owing to their conformational flexibility. To address this gap, we use a recently developed sensor based on Förster resonance energy transfer to measure steric pressure generated at membrane surfaces by the intrinsically disordered

SUBMITTER: Houser JR 

PROVIDER: S-EPMC9515369 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature