Obesity-induced miR-802 directly targets AMPK and promotes nonalcoholic steatohepatitis in mice.

ABSTRACT:

Objective

Obesity-associated nonalcoholic fatty liver disease (NAFLD) is a leading cause of liver failure and death. However, the pathogenesis of NAFLD and its severe form, nonalcoholic steatohepatitis (NASH), is poorly understood. The energy sensor, AMP-activated protein kinase (AMPK), has decreased activity in obesity and NAFLD, but the mechanisms are unclear. Here, we examined whether obesity-induced miR-802 has a role in promoting NASH by targeting AMPK. We also investigated whether miR-802 and AMPK have roles in modulating beneficial therapeutic effects mediated by obeticholic acid (OCA), a promising clinical agent for NASH.

Methods

Immunoblotting, luciferase assays, and RNA-protein interaction studies were performed to test whether miR-802 directly targets AMPK. The ro

SUBMITTER: Sun H 

PROVIDER: S-EPMC9515436 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature