Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments

ABSTRACT: Quantifying urease activity is an important task for Microbial Induced Calcite Precipitation research. A new urease activity microplate assay using a fluorescent pH indicator is presented. The method is also suitable for automated measurements during microbioreactor experiments. The assay reagent consists of the green fluorescent pH-indicator fluorescein, urea and a phosphate buffer. After sample addition, the microbial urease hydrolyses urea, which results in a pH and hence fluorescence increase. The fluorescence signal can be measured with a microplate reader or with the microbioreactor system BioLector, allowing for automated urease activity measurements during cultivation experiments. In both measurement systems, the fluorescence signal slope highly correlates with the urease activity

SUBMITTER: Lapierre F 

PROVIDER: S-EPMC9515450 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature