Characterization of a Trametes versicolor aflatoxin B1-degrading enzyme (TV-AFB1D) and its application in the AFB1 degradation of contaminated rice in situ.

ABSTRACT: Aflatoxin B₁ (AFB₁) contaminates rice during harvest or storage and causes a considerable risk to human and animal health. In this study, Trametes versicolor AFB₁-degrading enzyme (TV-AFB₁D) gene recombinantly expressed in engineered E. coli BL21 (DE3) and Saccharomyces cerevisiae. The TV-AFB₁D enzymatic characteristics and AFB₁ degradation efficiency in contaminated rice were investigated. Results showed that the size of recombinant TV-AFB₁D expressing in E. coli BL21 (DE3) and S. cerevisiae was appropriately 77 KDa. The kinetic equation of TV-AFB₁D was y = 0.01671x + 1.80756 (R ² = 0.994, K_m  = 9.24 mM, and V_m

SUBMITTER: Yang P 

PROVIDER: S-EPMC9515612 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature