P432 Detection of erg11 gene mutation in fluconazole resistant Candida albicans isolates

ABSTRACT: Abstract

Poster session 3, September 23, 2022, 12:30 PM - 1:30 PM

 

Objective

To determine the profile of fluconazole-resistant Candida isolates (FRCS) and the proportion of erg11 gene mutation in fluconazole-resistant C. albicans (FRCA) isolates

Methods

After getting approval from ethics the study was conducted on 150 isolates of FRCS obtained from patients admitted to our tertiary care hospital from September 2019 to December 2021. All the isolates were revived onto Sabouraud dextrose agar (SDA) and identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Fluconazole-resistance was detected by VITEK 2 automated system. Deoxyribonucleic acid (DNA) of FRCA isolates were extracted by QIAamp DNA Mini kit (Qiagen-51 

SUBMITTER:  

PROVIDER: S-EPMC9515968 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature