TRACES: a Freely Accessible, Semi-automated Pipeline for Detection, Tracking, and Quantification of Fluorescently Labeled Cellular Structures.

ABSTRACT: Subcellular structures exhibit diverse behaviors in different cellular processes, including changes in morphology, abundance, and relative spatial distribution. Faithfully tracking and quantifying these changes are essential to understand their functions. However, most freely accessible methods lack integrated features for tracking multiple objects in different spectral channels simultaneously. To overcome these limitations, we have developed TRACES (Tracking of Active Cellular Structures), a customizable and open-source pipeline capable of detecting, tracking, and quantifying fluorescently labeled cellular structures in up to three spectral channels simultaneously at single-cell level. Here, we detail step-by-step instructions for performing the TRACES pipeline, including image acquisitio

SUBMITTER: Jiang X 

PROVIDER: S-EPMC9516249 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature