METTL3 suppresses anlotinib sensitivity by regulating m⁶A modification of FGFR3 in oral squamous cell carcinoma.

ABSTRACT:

Background

N6-methyladenosine (m⁶A) is an abundant nucleotide modification in mRNA, but there were few studies on its role in cancer drug sensitivity and resistance. Anlotinib has been proved to have effective antitumor effects in oral squamous cell carcinoma (OSCC) in our previous study. Here, we sought to investigate the treatment target of anlotinib and the function and mechanisms of m⁶A modification in regulating anlotinib effect in OSCC.

Methods

Anlotinib treatment in a dose-dependent manner, western blotting, qRT-PCR and cell lost-of-function assays were used to study the treatment target of anlotinib in OSCC. RNA m⁶A dot blot assays, the m⁶A MeRIP-seq and MeRIP-qPCR, RNA and protein stability assays were used to explore the

SUBMITTER: Chen J 

PROVIDER: S-EPMC9516809 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature