Parasitic strongyle nemabiome communities in wild ruminants in Sweden.

ABSTRACT:

Background

Wildlife hosts may serve as reservoirs for strongyles, which can be transmitted to domestic livestock. Therefore, studies evaluating nemabiome compositions in wildlife ruminants are of great use in assessing the possibility of transmission of important nematode pathogens to domestic sheep in Sweden.

Methods

First, fecal samples were collected from roe deer (n = 125), fallow deer (n = 106), red deer (n = 18) and mouflon (n = 13) in south central Sweden during the hunting season in 2019. Second, after fecal examination samples were cultured and the larvae were harvested, followed by DNA extractions. Third, all samples were barcoded and processed for sequence analysis on the PacBio platform. Finally, bioinformatic sequence analysis was conducted with DADA2, while spe

SUBMITTER: Halvarsson P 

PROVIDER: S-EPMC9516825 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature