Shifts of Antibiotic Resistomes in Soil Following Amendments of Antibiotics-Contained Dairy Manure.

ABSTRACT: Dairy manure is a nutrition source for cropland soils and also simultaneously serves as a contamination source of antibiotic resistance genes (ARGs). In this study, five classes of antibiotics including aminoglycosides, beta-lactams, macrolides, sulfonamides, and tetracyclines, were spiked in dairy manure and incubated with soil for 60 days. The high throughput qPCR and 16S rRNA amplicon sequencing were used to detect temporal shifts of the soil antibiotic resistomes and bacterial community. Results indicated dairy manure application increased the ARG abundance by 0.5-3.7 times and subtype numbers by 2.7-3.7 times and changed the microbial community structure in soils. These effects were limited to the early incubation stage. Selection pressure was observed after the addition of sulfonamid

SUBMITTER: Kang J 

PROVIDER: S-EPMC9517759 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature