Specific histamine regulating activity of surface-modified yeast vacuoles by histamine- binding protein and its immune-enhancing effect.

ABSTRACT: We aimed to develop a biocompatible material that could enhance weakened immunity and control histamine in vivo. Histamine-binding protein (HBP) vacuoles have a mechanism of action that directly binds to the histamine molecule. It is designed to eliminate the side effects of antihistamine caused by binding to other receptors. HBP vacuoles were designed to produce a material that was biocompatible, and could enhance immunity. First, a recombinant vector was designed so that HBP was located on the vacuole surface, and expressed towards the cytoplasm. The vector was transformed into yeast, and protein expression was induced. Then, the vacuole was isolated by centrifugation to complete HBP vacuoles. Cytotoxicity test was conducted for application to RAW 264.7 cells. In addition, immune enhance

SUBMITTER: Jang H 

PROVIDER: S-EPMC9518974 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature