Investigating protein–excipient interactions of a multivalent VHH therapeutic protein using NMR spectroscopy

ABSTRACT: ABSTRACT Multispecific therapeutic proteins come in a variety of formats, including bi- and tri-specific antibodies, dual-variable domain antibodies, and CrossMabs. These multivalent proteins are engineered to interact with multiple therapeutic target proteins with high specificity. Multi-domain proteins can be created by linking together a variety of high-affinity antibody fragments. The choice of protein domains and linkers not only affects the interactions of these molecules with therapeutic targets but also influences the intrinsic behavior in solution that affects their stability. The complexity of solution interactions may translate into developability and manufacturing challenges. Here, we use nuclear magnetic resonance (NMR) spectroscopy to study the solution behavior of a multival

SUBMITTER: Panchal J 

PROVIDER: S-EPMC9519013 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature