Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues.
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ABSTRACT:
SUBMITTER: Martinek J
PROVIDER: S-EPMC9519630 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues.
Martinek Jan J Wu Te-Chia TC Sun Lili L Lin Jianan J Kim Kyung In KI Marches Florentina F Robson Paul P George Joshy J Palucka Karolina K
STAR protocols 20220922 4
We describe a pipeline for optimized and streamlined multiplexed immunofluorescence-guided laser capture microdissection allowing the harvest of individual cells based on their phenotype and tissue localization for transcriptomic analysis with next-generation RNA sequencing. Here, we analyze transcriptomes of CD3+ T cells, CD14+ monocytes/macrophages, and melanoma cells in non-dissociated metastatic melanoma tissue. While this protocol is described for melanoma tissues, we successfully applied i ...[more]
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