Successive mycelial subculturing decreased lignocellulase activity and increased ROS accumulation in Volvariella volvacea.
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ABSTRACT: Strain degradation is a common problem in many artificially-cultivated edible mushrooms. As a fungus with poor tolerance to low-temperature, Volvariella volvacea cannot delay its degradation by long-term low temperature storage like other fungi, so its degradation is particularly severe, which hinders industrial applications. Periodic mycelial subculture is a common storage method for V. volvacea, but excessive subculturing can also lead to strain degeneration. After 20 months of continuous subculturing every 3 days, V. volvacea strains S1-S20 were obtained, and their characteristics throughout the subculture process were analyzed. With increasing number of subculture, the growth rate, mycelial biomass, the number of fruiting bodies and biological efficiency gradually
SUBMITTER: Zhao F
PROVIDER: S-EPMC9520666 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
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