Novel angiotensin-converting enzyme and pancreatic lipase oligopeptide inhibitors from fermented rice bran.

ABSTRACT: This study determined the inhibitory activity of oligopeptides against angiotensin-converting enzyme (ACE) and pancreatic lipase through in vitro tests, molecular docking, and enzyme inhibition. The results showed that the IC₅₀ of GLLGY, HWP, and VYGF for ACE inhibition was 1 mg/mL, and the IC₅₀ of HWP for pancreatic lipase was 3.95 mg/mL. Molecular docking revealed that the binding energies between GLLGY, HWP, and VYGF and ACE were -9.0, -8.4, and -9.2 kcal/mol, respectively. The binding free energy between HWP and pancreatic lipase was -7.3 kcal/mol. GLLGY, HWP, and VYGF inhibited ACE compentitively. HWP inhibited pancreatic lipase through non-competition. in vitro simulated gastrointestinal digestion, the three oligopeptides still had inhibitory activ

SUBMITTER: Hu J 

PROVIDER: S-EPMC9520749 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature