Hyperosmolar expansion medium improves nucleus pulposus cell phenotype

ABSTRACT: Abstract

Background

Repopulating the degenerated intervertebral disc (IVD) with tissue‐specific nucleus pulposus cells (NPCs) has already been shown to promote regeneration in various species. Yet the applicability of NPCs as cell‐based therapy has been hampered by the low cell numbers that can be extracted from donor IVDs and their potentially limited regenerative capacity due to their degenerated phenotype. To optimize the expansion conditions, we investigated the effects of increasing culture medium osmolarity during expansion on the phenotype of dog NPCs and their ability to produce a healthy extracellular matrix (ECM) in a 3D culture model.

Methods

Dog NPCs were expanded in expansion medium with a standard osmolarity of 300 mOsm/L or adjusted to 400 or 500 mOsm/L in

SUBMITTER: Laagland L 

PROVIDER: S-EPMC9520765 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature