Proteomics profiling identifies extracellular vesicles’ cargo associated with tumour cell induced platelet aggregation

ABSTRACT:

Background

Cancer patients have an increased risk of developing venous thromboembolism, with up to 30% dying within a month of their development. Some cancer cells are known to induce platelet aggregation, and this interaction is understood to contribute to thrombosis and haematogenous metastasis. Many researchers have reported on extracellular vesicles (EVs) released from platelets. However, less is known about how cancer cells’ EVs may affect platelet function. Here EVs released by triple-negative breast cancer (TNBC) cell line variants were extensively investigated in this regard.

Methods

EVs were separated from conditioned media of TNBC Hs578T and Hs578Ts(i)8 cells using filtration and ultracentrifugation and were characterised by nanoparticle tracking analysis, immun

SUBMITTER: McNamee N 

PROVIDER: S-EPMC9520807 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature