Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae: A new protocol.

ABSTRACT: Invertebrates are becoming increasingly popular models for research on the immune system. The innate immunity possessed by insects shows both structural and functional similarity to the resistance displayed by mammals, and many processes occurring in insect hemocytes are similar to those that occur in mammals. However, the use of insects as research models requires the development of methods for working with hemocytes. The aim of this study was to develop a protocol for intracellular cytokine detection in Galleria mellonella larvae hemocytes based on flow cytometry. It describes the anticoagulant composition of the buffer, the optimal conditions for hemocyte permeabilization and fixation, as well as the conditions of cell centrifugation to prevent cell disintegration. A key element is the

SUBMITTER: Wronska AK 

PROVIDER: S-EPMC9521830 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature