A remodeled RNA polymerase II complex catalyzing viroid RNA-templated transcription.

ABSTRACT: Viroids, a fascinating group of plant pathogens, are subviral agents composed of single-stranded circular noncoding RNAs. It is well-known that nuclear-replicating viroids exploit host DNA-dependent RNA polymerase II (Pol II) activity for transcription from circular RNA genome to minus-strand intermediates, a classic example illustrating the intrinsic RNA-dependent RNA polymerase activity of Pol II. The mechanism for Pol II to accept single-stranded RNAs as templates remains poorly understood. Here, we reconstituted a robust in vitro transcription system and demonstrated that Pol II also accepts minus-strand viroid RNA template to generate plus-strand RNAs. Further, we purified the Pol II complex on RNA templates for nano-liquid chromatography-tandem mass spectrometry analysis and identifi

SUBMITTER: Dissanayaka Mudiyanselage SD 

PROVIDER: S-EPMC9521916 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature