Identification of putative enhancer-like elements predicts regulatory networks active in planarian adult stem cells.

ABSTRACT: Planarians have become an established model system to study regeneration and stem cells, but the regulatory elements in the genome remain almost entirely undescribed. Here, by integrating epigenetic and expression data we use multiple sources of evidence to predict enhancer elements active in the adult stem cell populations that drive regeneration. We have used ChIP-seq data to identify genomic regions with histone modifications consistent with enhancer activity, and ATAC-seq data to identify accessible chromatin. Overlapping these signals allowed for the identification of a set of high-confidence candidate enhancers predicted to be active in planarian adult stem cells. These enhancers are enriched for predicted transcription factor (TF) binding sites for TFs and TF families expressed in p

SUBMITTER: Neiro J 

PROVIDER: S-EPMC9522251 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature