Fine mapping of Rf5 region for a sorghum fertility restorer gene and microsynteny analysis across grass species.

ABSTRACT: Cytoplasmic male sterility (CMS) is widely used to control pollination in the production of commercial F₁ hybrid seed in sorghum. So far, 6 major fertility restorer genes, Rf1 to Rf6, have been reported in sorghum. Here, we fine-mapped the Rf5 locus on sorghum chromosome 5 using descendant populations of a 'Nakei MS-3A' × 'JN43' cross. The Rf5 locus was narrowed to a 140-kb region in BTx623 genome (161-kb in JN43) with 16 predicted genes, including 6 homologous to the rice fertility restorer Rf1 (PPR.1 to PPR.6). These 6 homologs have tandem pentatricopeptide repeat (PPR) motifs. Many Rf genes encode PPR proteins, which bind RNA transcripts and modulate gene expression at the RNA level. No PPR genes were detected at the Rf5 locus

SUBMITTER: Kiyosawa A 

PROVIDER: S-EPMC9522528 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature