Allelic variation of a clubroot resistance gene (Crr1a) in Japanese cultivars of Chinese cabbage (Brassica rapa L.).

ABSTRACT: Clubroot resistance (CR) is an important trait in Chinese cabbage breeding worldwide. Although Crr1a, the gene responsible for clubroot-resistance, has been cloned and shown to encode the NLR protein, its allelic variation and molecular function remain unknown. Here, we investigated the sequence variation and function of three Crr1a alleles cloned from six CR F₁ cultivars of Chinese cabbage. Gain-of-function analysis revealed that Crr1a^Kinami90_a isolated from the cv. 'Kinami 90' conferred clubroot resistance as observed for Crr1a^G004 . Because two susceptible alleles commonly lacked 172 amino acids in the C-terminal region, we investigated clubroot resistance in transgenic Arabidopsis harboring the chimeric Crr1a, in whic

SUBMITTER: Hatakeyama K 

PROVIDER: S-EPMC9522534 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature