Severe intraventricular hemorrhage causes long-lasting structural damage in a preterm rabbit pup model.

ABSTRACT:

Background

Intraventricular hemorrhage causes significant lifelong mortality and morbidity, especially in preterm born infants. Progress in finding an effective therapy is stymied by a lack of preterm animal models with long-term follow-up. This study addresses this unmet need, using an established model of preterm rabbit IVH and analyzing outcomes out to 1 month of age.

Methods

Rabbit pups were delivered preterm and administered intraperitoneal injection of glycerol at 3 h of life and approximately 58% developed IVH. Neurobehavioral assessment was performed at 1 month of age followed by immunohistochemical labeling of epitopes for neurons, synapses, myelination, and interneurons, analyzed by means of digital quantitation and assessed via two-way ANOVA or Student's t test.

SUBMITTER: Romantsik O 

PROVIDER: S-EPMC9522590 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature