A preclinical model of cutaneous melanoma based on reconstructed human epidermis.

ABSTRACT: Malignant melanoma is among the tumor entities with the highest increase of incidence worldwide. To elucidate melanoma progression and develop new effective therapies, rodent models are commonly used. While these do not adequately reflect human physiology, two-dimensional cell cultures lack crucial elements of the tumor microenvironment. To address this shortcoming, we have developed a melanoma skin equivalent based on an open-source epidermal model. Melanoma cell lines with different driver mutations were incorporated into these models forming distinguishable tumor aggregates within a stratified epidermis. Although barrier properties of the skin equivalents were not affected by incorporation of melanoma cells, their presence resulted in a higher metabolic activity indicated by an increase

SUBMITTER: Leikeim A 

PROVIDER: S-EPMC9522649 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature