Interplay of starch debranching enzyme and its inhibitor is mediated by Redox-Activated SPL transcription factor.

ABSTRACT: The germination process is of central importance across the cultivated species involving several key enzymes for mobilization of stored food reserves. Pullulanase (PUL), a starch-debranching enzyme, plays an important role in mobilizing stored endosperm food reserves during germination. Pullulanase inhibitor (PULI) hinders PUL's activity through an unknown mechanism. Barley has one PUL and two PULI genes. During the time-dependent processes of seed germination, only PULI-1 expression shows an antagonistic relationship with that of PUL. Our data have indicated that the expression of PULI-1 is modulated by SPL (Squamosa-promoter-binding Protein Like) transcription factors, known to be targeted by miR156. We show that the binding of recombinant HvSPL3 protei

SUBMITTER: Kadoll SK 

PROVIDER: S-EPMC9522876 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature