Efficient generation of mNeonGreen Plasmodium falciparum reporter lines enables quantitative fitness analysis.

ABSTRACT: CRISPR editing has enabled the rapid creation of fluorescent Plasmodium transgenic lines, facilitating a deeper understanding of parasite biology. The impact of genetic perturbations such as gene disruption or the introduction of drug resistance alleles on parasite fitness is typically quantified in competitive growth assays between the query line and a wild type reference. Although fluorescent reporter lines offer a facile and frequently used method to measure relative growth, this approach is limited by the strain background of the existing reporter, which may not match the growth characteristics of the query strains, particularly if these are slower-growing field isolates. Here, we demonstrate an efficient CRISPR-based approach to generate fluorescently labelled parasite lines us

SUBMITTER: Hoshizaki J 

PROVIDER: S-EPMC9523114 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature