Dataset Information

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus.

ABSTRACT:

Background

Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry.

Objectives

This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples.

Methods

A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test.

Results

The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies.

Conclusions

The spbELISA has practical applications in assessing the vaccination status of large pig herds.

SUBMITTER: Cao Y

PROVIDER: S-EPMC9523335 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus.

Cao Yuying Y Yuan Li L Yang Shunli S Shang Youjun Y Yang Bin B Jing Zhizhong Z Guo Huichen H Yin Shuanghui S

Journal of veterinary science 20220901 5

<h4>Background</h4>Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry.<h4>Objectives</h4>This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples.<h4>Methods</h4>A spbELISA method was developed based on the recombinant E2 expressed in <i>Escherichia coli</i>. The specificity of this esta ...[more]

PMID: 36174976

Similar Datasets

Project description:Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance of viremia and the inability to generate CSFV-specific immune responses in animals. These animals also showed a type I interferon blockade in the absence of clinical signs. In this study, we assessed the infection generated in 7-week-old CSFV PI wild boars after infection with the African swine fever virus (ASFV). The wild boars were divided in two groups and were infected with ASFV. Group A comprised boars who were CSFV PI in a subclinical form and Group B comprised pestivirus-free wild boars. Some relevant parameters related to CSFV replication and the immune response of CSFV PI animals were studied. Additionally, serum soluble factors such as IFN-α, TNF-α, IL-6, IL-10, IFN-γ and sCD163 were analysed before and after ASFV infection to assess their role in disease progression.After ASFV infection, only the CSFV PI wild boars showed progressive acute haemorrhagic disease; however, the survival rates following ASFV infection was similar in both experimental groups. Notwithstanding, the CSFV RNA load of CSFV PI animals remained unaltered over the study; likewise, the ASFV DNA load detected after infection was similar between groups. Interestingly, systemic type I FN-α and IL-10 levels in sera were almost undetectable in CSFV PI animals, yet detectable in Group B, while detectable levels of IFN-γ were found in both groups. Finally, the flow cytometry analysis showed an increase in myelomonocytic cells (CD172a+) and a decrease in CD4+ T cells in the PBMCs from CSFV PI animals after ASFV infection.Our results showed that the immune response plays a role in the progression of disease in CSFV subclinically infected wild boars after ASFV infection, and the immune response comprised the systemic type I interferon blockade. ASFV does not produce any interference with CSFV replication, or vice versa. ASFV infection could be a trigger factor for the disease progression in CSFV PI animals, as their survival after ASFV was similar to that of the pestivirus-free ASFV-infected group. This fact suggests a high resistance in CSFV PI animals even against a virus like ASFV; this may mean that there are relevant implications for CSF control in endemic countries. The diagnosis of ASFV and CSFV co-infection in endemic countries cannot be ruled out and need to be studied in greater depth.

Dataset Information

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus.

Background

Objectives

Methods

Results

Conclusions

Publications

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets