Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus.

ABSTRACT:

Background

Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry.

Objectives

This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples.

Methods

A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test.

Results

The spbELISA successfully detected the antibody levels in swine

SUBMITTER: Cao Y 

PROVIDER: S-EPMC9523335 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature