Immunopeptidome of hepatocytes isolated from patients with HBV infection and hepatocellular carcinoma.

ABSTRACT:

Background & aims

Antigen-specific immunotherapy is a promising strategy to treat HBV infection and hepatocellular carcinoma (HCC). To facilitate killing of malignant and/or infected hepatocytes, it is vital to know which T cell targets are presented by human leucocyte antigen (HLA)-I complexes on patient-derived hepatocytes. Here, we aimed to reveal the hepatocyte-specific HLA-I peptidome with emphasis on peptides derived from HBV proteins and tumour-associated antigens (TAA) to guide development of antigen-specific immunotherapy.

Methods

Primary human hepatocytes were isolated with high purity from (HBV-infected) non-tumour and HCC tissues using a newly designed perfusion-free procedure. Hepatocyte-derived HLA-bound peptides were identified by unbiased mass spectrometry (M

SUBMITTER: de Beijer MTA 

PROVIDER: S-EPMC9523389 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature