Ratiometric fluorescent Si-FITC nanoprobe for immunoassay of SARS-CoV-2 nucleocapsid protein.

ABSTRACT: Coronavirus disease 2019 (COVID-19) highlights the importance of rapid and reliable diagnostic assays for the management of virus transmission. Here, we developed a one-pot hydrothermal method to prepare Si-FITC nanoparticles (NPs) for the fluorescent immunoassay of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (N protein). The synthesis of Si-FITC NPs did not need post-modification, which addressed the issue of quantum yield reduction during the coupling reaction. Si-FITC NPs showed two distinct peaks, Si fluorescence at λ _em = 385 nm and FITC fluorescence at λ _em = 490 nm. In the presence of KMnO₄, Si fluorescence was decreased and FITC fluorescence was enhanced. Briefly, in the presence of N protein, cata

SUBMITTER: Mao G 

PROVIDER: S-EPMC9523638 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature