The heterogeneity of mammalian utricular cells over the course of development.

ABSTRACT:

Background

The inner ear organ is a delicate tissue consisting of hair cells (HCs) and supporting cells (SCs).The mammalian inner ear HCs are terminally differentiated cells that cannot spontaneously regenerate in adults. Epithelial non-hair cells (ENHCs) in the utricle include HC progenitors and SCs, and the progenitors share similar characteristics with SCs in the neonatal inner ear.

Methods

We applied single-cell sequencing to whole mouse utricles from the neonatal period to adulthood, including samples from postnatal day (P)2, P7 and P30 mice. Furthermore, using transgenic mice and immunostaining, we traced the source of new HC generation.

Results

We identified several sensory epithelial cell clusters and further found that new HCs arose mainly through differentia

SUBMITTER: You D 

PROVIDER: S-EPMC9523683 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature