Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast.

ABSTRACT:

Background

Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food. These methods rely on the detection of gene sequences to indicate the presence of viable bacteria. This indirect form of detection can be prone to experimental artefacts. Sample handling and processing are key sources of variation that require standard approaches. Extracting sufficient quantities of high quality DNA from food matrices is challenging because target bacterial species are usually minor components of the microbiota and foods contain an array of compounds that are inhibitory to downstream DNA applications. Here, three DNA extraction methods are compared for their ability to extract hig

SUBMITTER: Flint A 

PROVIDER: S-EPMC9524001 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature