A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems.

ABSTRACT: Cell-free gene expression (CFE) systems are an attractive tool for engineering within synthetic biology and for industrial production of high-value recombinant proteins. CFE reactions require a cell extract, energy system, amino acids, and DNA, to catalyse mRNA transcription and protein synthesis. To provide an amino acid source, CFE systems typically use a commercial standard, which is often proprietary. Herein we show that a range of common microbiology rich media (i.e., tryptone, peptone, yeast extract and casamino acids) unexpectedly provide an effective and low-cost amino acid source. We show that this approach is generalisable, by comparing batch variability and protein production in the following range of CFE systems: Escherichia coli (Rosetta^™ 2 (DE3), BL21(DE3)),

SUBMITTER: Nagappa LK 

PROVIDER: S-EPMC9524191 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature