Genomic insights into host and parasite interactions during intracellular infection by Toxoplasma gondii.

ABSTRACT: To gain insights into the molecular interactions of an intracellular pathogen and its host cell, we studied the gene expression and chromatin states of human fibroblasts infected with the Apicomplexan parasite Toxoplasma gondii. We show a striking activation of host cell genes that regulate a number of cellular processes, some of which are protective of the host cell, others likely to be advantageous to the pathogen. The simultaneous capture of host and parasite genomic information allowed us to gain insights into the regulation of the T. gondii genome. We show how chromatin accessibility and transcriptional profiling together permit novel annotation of the parasite's genome, including more accurate mapping of known genes and the identification of new genes and cis-regulatory elements. Mot

SUBMITTER: Ulahannan N 

PROVIDER: S-EPMC9524707 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature