Ca²⁺ channels couple spiking to mitochondrial metabolism in substantia nigra dopaminergic neurons.

ABSTRACT: How do neurons match generation of adenosine triphosphate by mitochondria to the bioenergetic demands of regenerative activity? Although the subject of speculation, this coupling is still poorly understood, particularly in neurons that are tonically active. To help fill this gap, pacemaking substantia nigra dopaminergic neurons were studied using a combination of optical, electrophysiological, and molecular approaches. In these neurons, spike-activated calcium (Ca²⁺) entry through Ca_v1 channels triggered Ca²⁺ release from the endoplasmic reticulum, which stimulated mitochondrial oxidative phosphorylation through two complementary Ca²⁺-dependent mechanisms: one mediated by the mitochondrial uniporter and another by the malate-aspartate shuttle. Di

SUBMITTER: Zampese E 

PROVIDER: S-EPMC9524841 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature