Human regulatory T cells locally differentiate and are functionally heterogeneous within the inflamed arthritic joint.

ABSTRACT:

Objective

Tregs are crucial for immune regulation, and environment-driven adaptation of effector (e)Tregs is essential for local functioning. However, the extent of human Treg heterogeneity in inflammatory settings is unclear.

Methods

We combined single-cell RNA- and TCR-sequencing on Tregs derived from three to six patients with juvenile idiopathic arthritis (JIA) to investigate the functional heterogeneity of human synovial fluid (SF)-derived Tregs from inflamed joints. Confirmation and suppressive function of the identified Treg clusters was assessed by flow cytometry.

Results

Four Treg clusters were identified; incoming, activated eTregs with either a dominant suppressive or cytotoxic profile, and GPR56⁺CD161⁺CXCL13⁺ Tregs. Pseudot

SUBMITTER: Lutter L 

PROVIDER: S-EPMC9525321 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature